Rink Amide AM Resin LL (100–200 Mesh): For C-Terminal Amide Peptide Synthesis
Rink Amide AM Resin LL (Low Loading, 100–200 mesh) is a premium solid-phase synthesis resin designed for Fmoc-based solid-phase peptide synthesis (SPPS) where a C-terminal amide (-CONH₂) is desired. It features a Rink amide linker attached to a polystyrene-divinylbenzene (PS-DVB) matrix, providing an acid-labile bond that releases the fully protected or deprotected peptide with an amidated C-terminus upon treatment with trifluoroacetic acid (TFA).
The "LL" indicates Low Loading, which is particularly useful for difficult sequences, long peptides, or peptidomimetics, helping to reduce aggregation and steric hindrance during synthesis.
Product Specifications
Matrix: Polystyrene cross-linked with 1% DVB
Linker: Rink amide (acid-labile, provides amide upon cleavage)
Loading: ~0.2–0.4 mmol/g (Low Loading)
Particle Size: 100–200 mesh (~75–150 µm)
Swelling: Excellent in DMF, DCM, NMP, and other polar aprotic solvents
Protecting Group: Fmoc (base-labile)
Key Features
C-Terminal Amide Peptides: Ideal for bioactive peptides that mimic natural amidation (common in hormones, neuropeptides, and therapeutic leads).
Low Loading (LL): Minimizes intermolecular interactions and aggregation, improving yield and purity in long or hydrophobic sequences.
Rink Amide Linker: Stable under basic conditions (e.g., piperidine deprotection) and cleavable under standard acidic conditions (e.g., TFA).
Excellent Swelling & Accessibility: Ensures effective reagent penetration and efficient coupling reactions.
Applications
Peptide Therapeutics Development: Suitable for peptides requiring a terminal amide to improve bioavailability or mimic natural structures.
Difficult or Aggregation-Prone Sequences: Low loading helps ensure better solubility and accessibility during synthesis.
Custom Peptide Libraries: Widely used in combinatorial chemistry and structure-activity relationship (SAR) studies.
Automated and Manual SPPS: Compatible with both synthesis methods.
Cleavage and Deprotection
Cleaved using TFA cocktails (e.g., 95% TFA, 2.5% water, 2.5% triisopropylsilane) for 1–3 hours at room temperature.
Yields peptides with fully deprotected side chains and a C-terminal amide.
Rink amide linker is stable during base treatments (e.g., Fmoc removal with 20% piperidine in DMF).
Handling and Storage
Store dry in an airtight container at room temperature.
Protect from light and moisture.
Pre-swell before use for optimal reactivity and resin performance.
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